Thursday, September 26, 2013

How Are Various Types Of Culture Media Used In Microbiology?

The controlled manner of cultivation of microorganisms with the help of predetermined culture media is known as Microbiological Culture. In this process bacteria or other microbiological organisms are grown or cultured so that their type can be identified and also for obtaining antigens which would be used in the production of vaccines. Other uses for culture media are genetic studies for manipulating the bacteria so that it does not affect or cause any harm.

It is important for a scientist to understand the cell structure and the nutritional values before any type of culture media is used to grow the organism. The cells structures are broken down by the scientists through which they get to know about the elements of which the bacteria are made of. Generally a bacterium is made up of sugars, Nucleic acids, Amino Acids and lipids. The chemical formulations of these compounds are derived according to which it is decided that what type of culture media would be used on the bacteria. Various Culture Media Manufacturers are out there who provide superior quality bacteria growth stimulating products.

The following Categories which differentiate the types of culture media used for various purposes:

Preservation Culture Media – as the name suggests the type of culture media in this category used for the preservation of microorganisms. The preservation Culture media comes in various forms – Solid, Liquid and semi-Liquid (jelly like). These medias compose all the necessary nutrients required for microbial growth specifically bacteria. The culture media in this category is capable of preserving bacteria for a long period of time so that any future examinations or tests can be carried out on the organism without any hassles.

Agar – this is a type of culture media which is mainly used for preparing growth agent which is in solid state. Most of the agar products are obtained from the strands of seaweeds. They do not contain any nutrition value. 2% agar is mixed with other medium to produce a culture which would be used on the microorganism. Agar melts at 98 degree Celsius and sets at 42 degree Celsius and does not get affected by the growth of the organism on which it is used. This is the most common type of growth stimulating ingredient which is used in almost every type of culture media as it is compatible to the nutritional values of every kind of bacteria.

Dehydrated Culture Media – this media contains a wide variety of ingredients like Agar and Broth in Vegetarian and non-vegetarian variants. Dehydrated culture media is widely used in microbiology for the purpose of research work, waste water treatment, veterinary sciences, Pharmaceutical companies, Diary and Food industries, Vaccine Production, Antibiotics production and insulin production. It is important that the Culture Media should be kept in a controlled environment so that it does not get stale and unfit for use on bacteria. It depends on each and every type of culture media that what temperature is required for its preservation.

Other Culture Media Types – Fermented culture media, isolated culture media, Resuscitation Culture media, differential culture media, Selective Culture Media, and Enrichment Culture Media. These include a huge variety of Culture Media Raw Material like Blood Agar, simple Agar, Agar jelly, Veg. Dehydrated culture media like Acetamide Broth, Starch Agar, Glucose, and Peptone Water which are used to stimulate growth among different types of bacteria and other microorganisms.

The manufacturers of these Culture media use the latest techniques to produce the finest quality of the Culture media product which is immensely effective in encouraging the growth of a bacteria or any other microorganism on which is used.

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